mitochondria, apoptosis, metacaspase

My younger sibling has taught me so many new things! With Helena I have learnt original and interesting ways to improve conversation engagement:

- There is no better pick-up line than: “Did you know that metacaspase is directly related to cellular apoptosis?

- Or, I can now go to my local bakery and suggest: “Did you know you should strengthen the use of Saccharomyces cerevisiae in order to enhance your current production?”

- Or even, when we have guests over for dinner and suddenly run out of conversation topics, I can always rescue the evening from certain boredom with: “How about those mitochondria proteins..? Aren’t they so essential to multicellular forms of life?”

    Anyways… what I am trying to say is congrats Helena on her hard work, endless sleepless nights and no social life for the past year it got her an 18 on her master’s thesis today!! Hurray!!


    1. It seems as if u studied ur lesson well!!!Those matacaspases, aconitases, and that dame programmed cellular death..that´s all very useful will come in handy someday..u´ll see...mark my words...
      But yes indeed, S.cerevisiae is a fine yeast to work with, simple, non expensive, a rapid life cycle, the whole genome has been sequenced…what more can u ask for?! metacaspase is the first know «caspase-like» protein in yeast cells and has been shown to be activated in diverse apoptotic stimuli!!!!(WOW!!!!) but no substrate has been described so far…(OH NO!!!!) indeed with my master’s thesis contribution , aconitase has been revealed as a possible metacaspase substrate..(WHO GIVES A DAME???) but I´ll leave hear the abstract of my thesis for u´r consultancy (THERE GO ALL UR BLOG READERS..)

      TITLE: Aconitase as a target of fragmentation during oxidative stress induced apoptosis in Saccharomyces cerevisiae


      It is nowadays consensual that yeast Saccharomyces cerevisiae has the basic molecular machinery needed to undergo cell death, with an apoptotic phenotype upon a wide range of external or physiological stimuli. Since the discovery of yeast metacaspase, Yca1p, a metacaspase-dependent apoptotic pathway has been revealed. However, despite the efforts to uncover the mechanisms involved in this apoptotic pathway little is known about the events upstream and downstream metacaspase activation.
      By combining digestome analysis with mass spectrometry analysis and in vivo assays, yeast aconitase-1 (Aco1p) has been identified as a target of Yca1p mediated fragmentation during hydrogen peroxide induced apoptosis. Additionally, our results demonstrate that yeast wild type cells present resistance to H2O2-induced apoptosis in the presence of fluoracetic acid, a specific inhibitor of aconitase enzymatic activity in the tricarboxylic acid cycle. Nevertheless, results suggest that, inhibition of Aco1p function in the maintenance of mtDNA, promoted by its Yca1p fragmentation, might be the greater contributor for H2O2-induced apoptosis. Furthermore, results indicate that aconitase is necessary for GAPDH cleavage in total cellular extracts and that mitochondrial localized GAPDH is not cleaved by Yca1p. Aconitase cleavage does not appear to influence the NO levels produced during H2O2-induced apoptosis.
      Altogether the results indicate that Aco1p is a target of Yca1p fragmentation during H2O2-induced apoptosis, and a link between its cleavage and aconitase functions impairment is suggested particularly in what concerns Aco1p function in mtDNA maintenance. However the exact mechanisms underlying Aco1p involvement in H2O2-induced apoptotic cell death remain to be elucidated.* END OF ABSTRACT (THANK GOD!!!)

      a portuguese version can be provided if wanted..(WHO THE HELL WANTS THAT????)

      ps. are those the best pictures u have of me???!!! I deserve this treatment?? It´s as if I filled ur blog with bowl shit on apoptosis, metacaspase and aconitase!!

      Helena Marques